In vitro Propagation to Conserve the Local Endemic and Endangered Medicinal Plant Helianthemum germanicopolitanum Bornm

Abstract In this study, in vitro propagation and acclimatization of Helianthemum germanicopolitanum Bornm. plant, a local endemic in Çankırı Province (Turkey) with arid and semi-arid lands, and an endangered species taking part among medicinal and aromatic plants were accomplished, which is under-researched. In this study, three basal media [a) Murashige and Skoog b) Gamborg's B5, and c) Nitsch & Nitsch], two gelling agents (agar 7 g/L, and gelrite 2.1 g/L), eight cytokinins and eight auxin doses of plant growth regulators [a) 6-benzyladenin, b) Kinetin-(0, 0.5, 1, and 2 mg/L), c) Indole-3-butyric acid, d) α-napthaleneacetic acid-(0, 0.25, 0.5, and 1 mg/L)] prepared in 64 different combinations with 30 g/L sucrose was added to the basal media and adjusted to pH 5.7 for in vitro propagation of H. germanicopolitanum. During in vitro propagation of the plant, external and internal infections were frequently encountered and this was solved by the developed protocol. The best shoot growth (1.141 cm) and shoot length (0.572 cm) were obtained in the Gamborg's B5 medium in combination with Kinetin (0.5 mg/L)+Indole-3-butyric acid (0.5 mg/L)+gelrite. The maximum number of shoots (19.50) and the best multiplication rate (94%) were obtained in the media containing benzyladenin (1 mg/L)+Indole-3-butyric acid (0.5 mg/L) plant growth regulator in Murashige and Skoog medium solidified with agar. At the rooting stage, the maximum number of roots (30) was reached in the Murashige and Skoog medium containing gelrite and the best rooting rate (92%) with agar. A hundred plants representing the best shoot and root growth were taken to acclimatization stage, and 32 of these plants adapted to external conditions.

Evaluation of soil properties and flora under afforestation and natural forest in semi-arid climate of central Anatolia.

Arid and semi-arid climates and antropojen effects have caused rapid deterioration of the natural resources in the watershed. The main objectives of the study were to determine the correlation between soil properties and flora and to examine some soil properties and floristic composition under natural forest and afforestration. The topographical, geological and stand viewing maps of the watershed belong to 1955 and 2006 years were eximaned in the study. According to this study, the 14.5% rate of black pine forest in 1955 increased to 35.8% in 2006. Degraded forest, degraded black pine and agricultural area decreased respectively to 5.7, 1.8 and 15.8% in 51 years. The richest families in the research area were Asteraceae, Fabaceae, Lamiaceae, Poaceae, Brassicaceae, Caryophyllaceae, Scrophulariaceae, Boraginaceae, Apiaceae and Rosaceae. The rate of the richest families was 71.75% in the total species, the remaining 22 families were scattered to 28.25% in proportion of species.